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ERX11599836: qiita_ptid_10331:12842.BLANK2.2H
1 ILLUMINA (Illumina MiSeq) run: 176 spots, 26,560 bases, 76,908b downloads

Design: Use of genetic and chemical biomarkers in the fecal material of pronghorn to understand demographic data.
Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)
Study: Using the metagenome and metabolome to explain links between human disturbances and demographics of pronghorn
show Abstracthide Abstract
Plants and the animals that eat them are in a constant battle for survival. Plants continue to produce new toxic chemicals while herbivores, and their associated microbial communities, battle back with genetic changes that provide tolerance to plant toxins. Low tolerance to toxins can alter habitat use by herbivores and result in loss of body mass, nutritional condition, and reproductive success. As such, the management of wild and domestic herbivores who compete for increasing limited plant resources across our changing landscapes could benefit from increased ability to manage toxin tolerance. This project seeks to use genetic and chemical biomarkers in the fecal material of pronghorn to understand demographic data. We will use genetics to identify diet composition and microbial communities in the gut and we will use metabolomics to assess the chemical profile of feces. Integrating the knowledge of diet composition, gut microbes, and the way in which herbivores absorb and metabolize nutrients and secondary metabolites from their plants will be modeled to predict survival of individuals.
Sample: Using the metagenome and metabolome to explain links between human disturbances and demographics of pronghorn; 12842.BLANK2.2H
SAMEA114572520 • ERS16610100 • All experiments • All runs
Organism: metagenome
Library:
Name: 12842.BLANK2.2H
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: Illumina EMP protocol 515fbc, 806r amplification of 16S rRNA V4
Experiment attributes: (show all 29 attributes...) (hide...)
barcode: CGCCGTACTTGC
center_name: UCSDMI
center_project_name: Pronghorn
extraction_robot: Carmen_HOWE_KF_4
extractionkit_lot: 163051748
linker: GT
mastermix_lot: 978215
orig_name: BLANK2.2H
pcr_primers: FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT
plating: MB
primer: GTGTGYCAGCMGCCGCGGTAA
primer_date: 62320
primer_plate: 2
processing_robot: Echo550
project_name: Hayden BSU Herbivore Reruns
run_center: UCSDMI
run_date: 2/12/21
run_prefix: Hayden_BSU_Herbivore_Reruns_Plate_1-5_w_DHMC_Mills (show full text...)(hide...)
Hayden_BSU_Herbivore_Reruns_Plate_1-5_w_DHMC_Mills_Plate_2_S1_L001
runid: 210212_M05314_0275_000000000_JGYD5
sample_plate: Hayden_BSU_Herbivore_Reruns_Plate_2
sequencing_meth: Sequencing by synthesis
target_gene: 16S rRNA
target_subfragment: V4
tm1000_8_tool: NA
tm300_8_tool: NA
tm50_8_tool: NA
water_lot: RNBJ0628
well_description: Hayden_BSU_Herbivore_Reruns_Plate_2_BLANK2.2H_H2
well_id: H2
Runs: 1 run, 176 spots, 26,560 bases, 76,908b
Run# of Spots# of BasesSizePublished
ERR1218896717626,56076,908b2024-07-20

ID:
33886545

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